Electrochemical Study and Characterization of an Amperometric Biosensor Based on the Immobilization of Laccase in a Nanostructure of TiO2 Synthesized by the Sol-Gel Method
نویسندگان
چکیده
Laccase amperometric biosensors were developed to detect the catechol compound. The laccase enzyme (LAC) immobilization was performed on nanostructures of (a) titania (TiO₂); (b) titania/Nafion (TiO₂/NAF) (both immobilized by the sol-gel method) and a third nanostructure, which consisted of a single biosensor composite of Nafion and laccase enzyme denoted as NAF/LAC. The Nafion was deposited on a graphite electrode and used to avoid "cracking" on the matrix. The TiO₂ particle size was an average of 66 nm. FTIR spectroscopy vibration modes of different composites were determined. The electrochemical behavior of the biosensor was studied using electrochemical spectroscopy (EIS) and cyclic voltammetry (CV). The biosensor based on TiO₂/NAF/LAC presented the best electro-chemical properties with regard to sensitivity, stability and detection limit after a period of 22 days.
منابع مشابه
Synthesis and characterization of Ag-doped TiO2 nanostructure and investigation of its application as dye-sensitized solar cell
A new strategy for enhancing the efficiency of TiO2 dye-sensitized solar cells (DSSCs) by doping foreign ion into TiO2 lattice via sol-gel process is reported. DSSCs are based on a semiconductor (i.e., TiO2), formed between a photo-sensitized anode and an electrolyte. In order to reach high conversion efficiency, it is important to increase the electron injection and optical absorption. One pro...
متن کاملSynthesis and Characterization of Doped TiO2 Nanoparticles by the Sol-Gel Method and Investigation of Photocatalytic Activity
Cu2+,Ce3+-doped nanostructure TiO2 -coated Nd3+ (TiO2 /Nd3+) were prepared by sol-gel method. TiO2 were synthesized by the sol-gel method using tetra n-butyl orthotitanat (TBT) as a precursor. The prepared nanoparticles characterized by FT-IR, XRD, SEM- EDS and TEM. The XRD showed that the major phase of nanocomposite is anatase. The morphology of the TiO2/Nd3+/Cu2+and TiO2 /Nd3+/Ce3+ microsphe...
متن کاملAn investigation on the effect of acetone and DMF as solvent on synthesis of P2O5-CaO-Na2O-TiO2 glass powder by sol-gel method
The sol-gel synthesis method allows greater control over glass morphology at a relatively low processing temperature (200 °C) in comparison with melt-derived glasses. In present study, phosphate-based glasses with the general formula of (P2O5)55-(CaO)25-(Na2O)10-(TiO2)10 was synthesized via a novel and facil...
متن کاملSynthesis and nano structural study on TiO2-NiO-SiO2 composite
We report on the synthesis, morphology, chemically and structurally of TiO2-NiO-SiO2 nanostructure. The TiO2-NiO-SiO2 nanostructure was synthesized by a method based on the sol-gel method, by the simultaneous gelation of all cations. The coatings were deposited on dried soda-lime glass slides by spin coating. Composite powders and coating on glass hav...
متن کاملSynthesis and nano structural study on TiO2-NiO-SiO2 composite
We report on the synthesis, morphology, chemically and structurally of TiO2-NiO-SiO2 nanostructure. The TiO2-NiO-SiO2 nanostructure was synthesized by a method based on the sol-gel method, by the simultaneous gelation of all cations. The coatings were deposited on dried soda-lime glass slides by spin coating. Composite powders and coating on glass hav...
متن کامل